ماتریکس سلول زدایی شده ریه و نقش آن به عنوان داربستی در شرایط آزمایشگاهی تمایز سلول‌های بلاستمایی

Background: Increasing number of patients facing end-organ failure, as well as the therapeutic challenges surrounding allotransplantation, has catalyzed the evolution of tissue engineering and regenerative medicine. The successful recapitulation of development requires choosing an ideal scaffold material as a mediator of biochemical and biophysical signals. The extracellular matrix (ECM) functions as a scaffold for tissue morphogenesis and provides cues for cell proliferation and differentiation. Rabbit decellularized lung tissue was used for fabrication of scaffold.                Method: In this experimental study, the fabrication of scaffold from rabbit lung tissue was done via snap freezing method, use of sodium dodecyl sulfate (SDS) and cell bleaching. Interactions between scaffold and blastema tissue were evaluated and scaffolds were placed in blastema rings and stored in culture media for 30 days. Sampling of blastema and scaffolding tissues was done for every five days.             Result: Histological and microscopic analysis confirmed the removal of cells from prepared scaffolds and porosity was done for lung tissue scaffold. The penetration, proliferation and differentiation of cells was also studied when blastema tissues were cultured on scaffold by using different days          Conclusion: In this study, a three-dimensional natural scaffold was fabricated from lung tissue using SDS treatment. The prepared scaffolds can have an inductive effect on the cell behaviors such as migration, penetration, division, and probable differentiation. However, further studies are necessary to demonstrate the identity and behavior of the cells and scaffold characterization. It seems obtained scaffold after decellularization of lung tissue can be used as suitable model for primary research in lung tissue engineering